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KMID : 1161520000040040389
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Animal Cells and Systems
2000 Volume.4 No. 4 p.389 ~ p.394
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Transeriptional regulation and apoptosis induction by TCF/β-catenin complex in various t-cells
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Jeong Sun-Joo
Lee Seung-Yeon
Lee Sun-Hee
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Abstract
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The Tcf?1 (T?cell factor?1) protein binds to the T?cell specific enhancer sequences and plays an architectural role in the assembly of transe riptio nal machinery. One of the Tcf family proteins, Tcf?4, was found to be an important regulator for colon cancer development where it activates specific genes upon binding to β?catenin following Wnt signaling. We were interested in the transe riptio nal regulatory activities of Tcf?1 and Tcf?4 proteins in T?cells and colon cancer cells. Trans activation assay was developed using a reporter plasmid containing luciferase gene under the control of Tcf responsive elements. Luciferase activity was determined following co?transfection of the reporter along with Tcf?1 and/or β?catenin expressing plasmids. Transcription was significantly induced by β?catenin expression in all cells. Tcf?1 by itself did not induce transcription in the mature T?cell lines, but overexpressed Tcf?1 greatly activated transcription in the immature T?cell line. In addition, transfected β?catenin induced apoptosis, but co?transfected Tcf?1 suppressed apoptosis in HEK293 cells. These results suggest that Tcf?1 and β?catenin differently regulate transcription and apoptosis.
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KEYWORD
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Tcf-1, Tcf-4, β-catenin, Transcription, Apoptosis, T-cell lines, HEK293 cell
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